2-Methylpropionate is a vitamin-B derivative also called thiamine disulfide. It is used in medicine for treatment of asthenia (weakness, loss of appetite and inability to concentrate). It is used as supplement by bodybuilders and athletes because it is believed to increase physical resistance to fatigue, improve neuromuscular efficiency, learning and memory, and improve metabolic function of the cerebral cortex. Some users have also reported improved sleep patterns after use of 2-methylpropionate supplements. Research shows that it improves energy levels by increasing choline uptake by hippocampal neurons, thus resulting in an increase in synthesis and release of acetylcholine. Its effects on brain are because it increases neuronal membrane permeability.

Caffeine is a powerful and very common alkaloid supplement used in Biotest Spike primarily to increase the body’s ability to lose weight quickly, boost energy and stimulate the brain into more activity with better clarity. It also fuels the body’s metabolism for increased fat burning qualities. This allows users to have more stamina for exercise and more focus for weight loss initiatives. Caffeine anhydrous is found naturally in many different beans and plants and works successfully as a stimulating supplement for those needing increased energy, focus, and alertness. When taken as a supplement for health or weight loss reasons, caffeine anhydrous should not be combined with other traditional forms of caffeine, such as soft drinks, energy drinks, coffee, or tea, because the supplement form is concentrated and powerful in itself.

Guarana Extract is the extract of the guarana plant. It contains a significant amount of guaranine, a substance that is similar to caffeine found in coffee beans. It helps stimulate the adrenal glands and increase the production and release of hormones such as epinephrine, norepinephrine (adrenaline), and dopamine. This helps the metabolism of the body that enhances fat loss, and increases energy and endurance. It helps the body burn the fats to be used for energy. It aids in fighting stress, weakness and fatigue. As a stimulant, it also improves mental alertness, clarity, focus, concentration, and memory retention. Guarana also also improves the libido or sex drive in both men and women. Guarana also contains tannins that help prevent diarrhea and soothes the digestive system.

Thiamine is a water-soluble vitamin with no known toxic effects if taken with other members of the vitamin B complex. Thiamine is essential to a strong appetite and to enable the body to use sugars and other carbohydrates.

Yohimbine HCL raises body temperatures. it increases the flow of blood to the genital area and increases the sex drive in men but also women have reported similar effects.