2-Methylpropionate is a vitamin-B derivative also called thiamine disulfide. It is used in medicine for treatment of asthenia (weakness, loss of appetite and inability to concentrate). It is used as supplement by bodybuilders and athletes because it is believed to increase physical resistance to fatigue, improve neuromuscular efficiency, learning and memory, and improve metabolic function of the cerebral cortex. Some users have also reported improved sleep patterns after use of 2-methylpropionate supplements. Research shows that it improves energy levels by increasing choline uptake by hippocampal neurons, thus resulting in an increase in synthesis and release of acetylcholine. Its effects on brain are because it increases neuronal membrane permeability.

Caffeine is of course a major component of every day drinks such as tea and coffee and is also included in Biotest Spike. It is claimed that over 70% of the American population take more than 300 milligrams daily. As well as in familiar drinks it is used in energy drinks such as Red Bull as well as in many over the counter analgesics and other medication. Medically it is a as trimethylxanthine, useful as a heart stimulant and it also works as a mild diuretic. It works in the same way as certain drugs, the amphetamines, cocaine, and heroin, all of which work on the action of the brain although in a milder way, and like them it is addictive.

Guarana Extract is the extract of the guarana plant. It contains a significant amount of guaranine, a substance that is similar to caffeine found in coffee beans. It helps stimulate the adrenal glands and increase the production and release of hormones such as epinephrine, norepinephrine (adrenaline), and dopamine. This helps the metabolism of the body that enhances fat loss, and increases energy and endurance. It helps the body burn the fats to be used for energy. It aids in fighting stress, weakness and fatigue. As a stimulant, it also improves mental alertness, clarity, focus, concentration, and memory retention. Guarana also also improves the libido or sex drive in both men and women. Guarana also contains tannins that help prevent diarrhea and soothes the digestive system.

Thiamine, also called Vitamin B1 or Thiamin, is needed to help the body utilize energy from proteins, fats and carbohydrates. Thiamine is commonly found in breads and cereals. Thiamine is water-soluble and is concentrated in muscle tissue as it passes thru the body.

Yohimbine HCL, also known as 17-hydroxy-yohimban. Yohimbine is an odorless; white crystalline powder; scarcely soluble in water; soluble in chloroform and alcohol. The chemical title is 17-hydroxy-yohimban-16- carboxylic acid, methyl ester. Yohimbine works as a weak blocker of alpha-adrenergic receptor. It is used in angina pectoris and arteriosclerosis, and has been used as a mydriatic and local anesthetic and for its supposed aphrodisiac properties. Yohimbine is a therapeutic derivative of the bark of a tree named Corynanthe yohimbine.